dna shearing sonication
Most probe-sonicators can be quite variable and require careful calibration to achieve the correct size-distribution. Samples of purified DNA are sheared into short fragments using either mechanical methods eg ultrasonication shearing and nebulization or enzymatic digestion 2. 5 Methylcytosine 5 Mc Monoclonal Antibody 33d3 Epigenetics Dna Methylation Mcs This technique uses acoustic cavitation to fragment DNA. . Requires relatively large amounts of DNA 10-100 mg. Acoustic shearing and sonication are the main physical methods used to shear DNA. Bioruptor 05 ml Microtubes for DNA Shearing Cat. Add 2-3ul 6x loading dye and load 6ul onto 12 pre-cast agarose gel. The susceptibility of nucleic acids to shearing can also benefit operators. Bacterial Genomic DNA Protocol. TE 10 mM Tris 1mM EDTA pH 75 - 80 DNA concentration1-20 ngµl 10 ngµl recommended Samples are vortexed 10-15 sec and centrifuged 10 sec before sh...